Orientation and Integrity of Plasma Membrane Vesicles Obtained from Carrot Protoplasts
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چکیده
منابع مشابه
Protoplasts Obtained from Candida tropicalis
A method for the preparation of protoplasts from Candida tropicalis cultivated on n-tetradecane is described. This essentially consists of replacing the mannitolsorbitol solution of the classical helicase technique by 1 M magnesium sulfate and lowering the pH to 4.1 during incubation in the presence of helicase. The protoplasts thus prepared behave like intact cells and are capable of consuming...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1983
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.73.2.385